BtGlu97A

Ex-Glu0004

(EC.3.2.1.20) exo-α-Glucosidase

CAZy Family: GH97

PROPERTIES

1.ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~76kDa)

Figure 1. Electrophoresis analysis of BtGlu97A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BtGlu97A purified from Ni sepharose fastflow column.

2.SPECIFIC ACTIVITY

5 U/mg protein (on pNP-α-glu) at pH7.0 and 37°C

One Unit of pNP-α-glu activity is defined as the amount of enzyme required to release 1 μmol of glucose per minute from pNP-α-glu (5 mM) in phosphate buffer(10 mM) pH7.0.

3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Table 1. Relative activity of BtGlu97A on different substrates^a.

5.STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (10 mM) pH7.0. Swirl to mix the enzyme immediately prior to use.