BfMan92A
Ex-Man0085
(EC.3.2.1.113)exo-α-1,2-Mannosidase
CAZy Family: GH92
PROPERTIES
1.ELECTROPHORETIC PURITY
-Single band on SDS-gel electrophoresis (MW ~86 kDa)
Figure 1. Electrophoresis analysis of BfMan92A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BfMan92A. purified from Ni sepharose fastflow column.
2.SPECIFIC ACTIVITY
0.29 U/mg protein (on pNP-α-Man) at pH 7.0 and 37oC.
One Unit of pNP-α-Man activity is defined as the amount of enzyme required to release 1 umole of p-nitrophenyl per minute from pNP-α-Man (5 mM) in PBS buffer (20 mM) pH 7.0.
3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES
Table 1. Relative activity of BfMan92A. on different substratesa.
Substrateb |
Relative activity (%)c |
pNPβGlu |
_ |
pNPβGal |
_ |
pNPβMan |
_ |
pNPβXyl |
_ |
pNPαGlc |
_ |
pNPαGal |
_ |
pNPαMan |
100%±0.0 |
pNPαAraf |
_ |
pNPαArap |
_ |
aReactions were performed with 5 mM substrate, pH 7.0, at 37°C for 10 min.
bAbsorption caused by released p-nitrophenol was measured at 405 nm.
cThe data are reported as means±standard errors from the mean for three independent experiments.
4.PHYSICOCHEMICAL PROPERTIES
pH Optima: 7.0
Temperature Optima: 37°C
5.STORAGE CONDITIONS
The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH7.0. Swirl to mix the enzyme immediately prior to use.
BfMan92A
Ex-Man0085
(EC.3.2.1.113)exo-α-1,2-Mannosidase
CAZy Family: GH92
PROPERTIES
1.ELECTROPHORETIC PURITY
-Single band on SDS-gel electrophoresis (MW ~86 kDa)
Figure 1. Electrophoresis analysis of BfMan92A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BfMan92A. purified from Ni sepharose fastflow column.
2.SPECIFIC ACTIVITY
0.29 U/mg protein (on pNP-α-Man) at pH 7.0 and 37oC.
One Unit of pNP-α-Man activity is defined as the amount of enzyme required to release 1 umole of p-nitrophenyl per minute from pNP-α-Man (5 mM) in PBS buffer (20 mM) pH 7.0.
3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES
Table 1. Relative activity of BfMan92A. on different substratesa.
Substrateb |
Relative activity (%)c |
pNPβGlu |
_ |
pNPβGal |
_ |
pNPβMan |
_ |
pNPβXyl |
_ |
pNPαGlc |
_ |
pNPαGal |
_ |
pNPαMan |
100%±0.0 |
pNPαAraf |
_ |
pNPαArap |
_ |
aReactions were performed with 5 mM substrate, pH 7.0, at 37°C for 10 min.
bAbsorption caused by released p-nitrophenol was measured at 405 nm.
cThe data are reported as means±standard errors from the mean for three independent experiments.
4.PHYSICOCHEMICAL PROPERTIES
pH Optima: 7.0
Temperature Optima: 37°C
5.STORAGE CONDITIONS
The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH7.0. Swirl to mix the enzyme immediately prior to use.
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