BcMan76A (endo-α-1,6-Mannanase)


BcMan92J

Endo-Man0103

(EC.3.2.1.101)endo-α-1,6-Mannanase

CAZy Family: GH76


PROPERTIES

1.ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~66kDa)


Figure 1. Electrophoresis analysis of BcMan76A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BcMan76A. purified from Ni sepharose fastflow column.

2.SPECIFIC ACTIVITY



3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES



4.PHYSICOCHEMICAL PROPERTIES

pH Optima: 7.0

Temperature Optima: 37°C


5.STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH7.0. Swirl to mix the enzyme immediately prior to use.

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BcMan76A (endo-α-1,6-Mannanase)


BcMan92J

Endo-Man0103

(EC.3.2.1.101)endo-α-1,6-Mannanase

CAZy Family: GH76


PROPERTIES

1.ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~66kDa)


Figure 1. Electrophoresis analysis of BcMan76A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BcMan76A. purified from Ni sepharose fastflow column.

2.SPECIFIC ACTIVITY



3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES



4.PHYSICOCHEMICAL PROPERTIES

pH Optima: 7.0

Temperature Optima: 37°C


5.STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH7.0. Swirl to mix the enzyme immediately prior to use.