BfMan92C

En-Man0087

(EC.3.2.1.113)exo-α-1,2-mannosidase

CAZy Family: GH92

PROPERTIES

1.ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~84kDa)

Figure 1. Electrophoresis analysis of BfMan92C. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BfMan92C. purified from Ni sepharose fastflow column.

2.SPECIFIC ACTIVITY

3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Table 1. Relative activity of BfMan92C. on different substrates^a.

^aReactions were performed with 5 mM substrate, pH 7.0, at 37°C for 10 min.

^bAbsorption caused by released p-nitrophenol or reducing sugars was measured at 405 or 520 nm.

^cThe data are reported as means±standard errors from the mean for three independent experiments.

4.PHYSICOCHEMICAL PROPERTIES

pH Optima: 7.0

Temperature Optima: 37°C

5.STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH7.0. Swirl to mix the enzyme immediately prior to use.