BtMan125A

Ex-Man0081

(EC.3.2.1.) exo-α-Mannosidase

CAZy Family: GH125

PROPERTIES

ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~55 kDa)

Figure 1. Electrophoresis analysis of BtMan125A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BtMan125A purified from Ni sepharose fastflow column.

PHYSICOCHEMICAL PROPERTIES

pH Suggestion: 7.0

Temperature Suggestion: 37°C

STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH7.0. Swirl to mix the enzyme immediately prior to use.