BvMan92F
Ex-Man0122
(EC.3.2.1.24) exo-α-Mannosidase
CAZy Family: GH92
PROPERTIES
1.ELECTROPHORETIC PURITY
-Single band on SDS-gel electrophoresis (MW ~86kDa)
Figure 1. Electrophoresis analysis of BvMan92F. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BvMan92F purified from Ni sepharose fastflow column.
2.SPECIFIC ACTIVITY
0.4 U/mg protein (on pNP-α-Man) at pH 7.0 and 37°C
One Unit of pNP-α-Man activity is defined as the amount of enzyme required to release 1 μmol of p-nitrophenyl per minute from pNP-α-Man (5 mM) in PBS buffer (20 mM) pH 7.0.
3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES
Table 1. Relative activity of BvMan92F on different substrates.
Substrate |
Relative activity (%) |
pNPβGlu |
_ |
pNPβGal |
33±0 |
pNPβMan |
_ |
pNPβXyl |
_ |
pNPαGlu |
_ |
pNPαGal |
_ |
pNPαMan |
100±0 |
pNPαAraf |
_ |
pNPαArap |
_ |
4.PHYSICOCHEMICAL PROPERTIES
pH Suggestion: 7.0
Temperature Suggestion: 37°C
5.STORAGE CONDITIONS
The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH7.0. Swirl to mix the enzyme immediately prior to use.
BvMan92F
Ex-Man0122
(EC.3.2.1.24) exo-α-Mannosidase
CAZy Family: GH92
PROPERTIES
1.ELECTROPHORETIC PURITY
-Single band on SDS-gel electrophoresis (MW ~86kDa)
Figure 1. Electrophoresis analysis of BvMan92F. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BvMan92F purified from Ni sepharose fastflow column.
2.SPECIFIC ACTIVITY
0.4 U/mg protein (on pNP-α-Man) at pH 7.0 and 37°C
One Unit of pNP-α-Man activity is defined as the amount of enzyme required to release 1 μmol of p-nitrophenyl per minute from pNP-α-Man (5 mM) in PBS buffer (20 mM) pH 7.0.
3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES
Table 1. Relative activity of BvMan92F on different substrates.
Substrate |
Relative activity (%) |
pNPβGlu |
_ |
pNPβGal |
33±0 |
pNPβMan |
_ |
pNPβXyl |
_ |
pNPαGlu |
_ |
pNPαGal |
_ |
pNPαMan |
100±0 |
pNPαAraf |
_ |
pNPαArap |
_ |
4.PHYSICOCHEMICAL PROPERTIES
pH Suggestion: 7.0
Temperature Suggestion: 37°C
5.STORAGE CONDITIONS
The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH7.0. Swirl to mix the enzyme immediately prior to use.
Copyright 2010--2022 © 东北师范大学 生命科学学院 All Rights Reserved
地址:吉林省长春市人民大街5268号 邮编:130024 电话:0431-85099453