BtAra51A

Ex-Ara0134

(EC.3.2.1.55) exo-α-Arabinofuranosidase

CAZy Family: GH51

PROPERTIES

1.ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~54 kDa)

2.SPECIFIC ACTIVITY

3.53 U/mg protein (on pNP-α-araf) at pH 6.0 and 37^oC

One Unit is defined as the amount of enzyme required to release 1 μmol of pNP per minute from pNP-α-araf (5 mM) in Sodium acetate buffer (20 mM) pH 6.0.

3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Table 1 Substrate specificity of BtAra51A

^aReactions were performed with 5 mM substrate, pH 6.0, at 37°C for 5 min.

^bAbsorption caused by released p-nitrophenol or reducing sugars was measured at 405 nm. The relative activity on pNPαAraf  was taken as 100%, respectively.

^cThe data are reported as means±standard errors from the mean for three independent experiments

^aReactions were performed with 10 μg substrate, pH 6.0, at 37°C for 24 h.

^bThe green five pointed star represents arabinose and the orange five pointed star represents xylose. The results show that BtAra51A(BT0348) can hydrolyze any kind of bond linked α-ara.

4.PHYSICOCHEMICAL PROPERTIES

pH Optima: 7.5

Temperature Optima: 37°C

5.STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (50 mM) pH 6.0.Swirl to mix the enzyme immediately prior to use.

6. REFERENCES

[1] Ana S. Luis, Jonathon Briggs, Xiaoyang Zhang, et al. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides [J] Nat Microbiol. 2018,3(2): 210–219.