BfAra43B

Endo-Gal0157

(EC. 3.2.1) exo-α-Arabinofuranosidase

CAZy Family: GH43

PROPERTIES

ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~34 kDa)

Figure 1. Electrophoresis analysis of BfAra43B. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BfAra43B purified from Ni sepharose fastflow column.

RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Table 1. Relative activity of BfAra43B on different substrates^a.

^aReactions were performed with 5 mM substrate, pH 7.0, at 37°C for 5 min.

^bAbsorption caused by released p-nitrophenol was measured at 405 nm. The relative activity on pNPαAraf was taken as 100%.

^cThe data are reported as means±standard errors from the mean for three independent experiments.

PHYSICOCHEMICAL PROPERTIES

pH Optima: suggested use 7.0

Temperature Optima: suggested use 37°C

STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.