PpAra43A

Endo-Ara0242

(EC.3.2.1.99) endo-α-1,5-L-Arabinanases

CAZy Family: GH43

PROPERTIES

1. ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~88 kDa)

Figure 1. Electrophoresis analysis of PpAra43A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, PpAra43A purified from Ni sepharose fastflow column.

2. SPECIFIC ACTIVITY

60.6 U/mg protein (on linear arabinan) at pH6.0 and 30°C.

One Unit of Arabinanases activity is defined as the amount of enzyme required to release 1 μmol of reducing sugar per minute from linear 1,5-α-L-arabinan in acetate buffer (20 mM) pH 6.0.

3. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Figure 2. HPAEC chromatogram showing enzymatic products of linear arabinan with PpAra43A. A1, arabinose; A2, arabinobiose; A3, arabinotriose; A4, arabinotetraose.

4. PHYSICOCHEMICAL PROPERTIES

pH Optima: 6.0

pH Stability: 5.0-7.0

Temperature Optima: 30°C

Temperature Stability: < 40°C

5. STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in acetate buffer (20 mM) pH 6.0. Swirl to mix the enzyme immediately prior to use.

6. REFERENCES

[1] Wang et al. Characterization of abnZ2 (yxiA1) and abnZ3 (yxiA3) i n Paenibacillus polymyxa, encoding two novel endo-1,5-α-L-arabinanases Bioresources and Bioprocessing 2014, 1:14