PpGal42C
Exo-Gal0251
(EC.3.2.1.23)exo-β-Galactosidase
CAZy Family: GH42
PROPERTIES
1.ELECTROPHORETIC PURITY
-Single band on SDS-gel electrophoresis (MW ~77.16 kDa)
Figure 1. Electrophoresis analysis of PpGal42C. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, PpGal42C purified from Ni sepharose fastflow column.
2.SPECIFIC ACTIVITY
1.24 U/mg protein (on pNP-β-gal) at pH 6.0 and 30°C
One Unit of pNP-β-gal activity is defined as the amount of enzyme required to release 1 μmol of p-nitrophenyl per minute from pNP-β-gal (5 mM) inacetate buffer (20 mM) pH 6.0.
3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES
Table 1. Relative activity of PpGal42C on different substratesa.
Substrateb |
Relative activity (%)c |
pNPβGlu |
_ |
pNPβGal |
100.0±0.6 |
pNPβMan |
_ |
pNPβXyl |
_ |
pNPαGlc |
_ |
pNPαGal |
_ |
pNPαMan |
_ |
pNPαAraf |
_ |
pNPαArap |
94.3±0.6 |
aReactions were performed with 5 mM substrate, pH 6.0, at 30°C for 5 min.
bAbsorption caused by released p-nitrophenol was measured at 405 nm. The relative activity on pNPβgal was taken as 100%.
cThe data are reported as means±standard errors from the mean for three independent experiments.
4.PHYSICOCHEMICAL PROPERTIES
pH Optima: 6.0
Temperature Optima: 30°C
5.STORAGE CONDITIONS
The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in acetate buffer (20 mM) pH 6.0. Swirl to mix the enzyme immediately prior to use.
PpGal42C
Exo-Gal0251
(EC.3.2.1.23)exo-β-Galactosidase
CAZy Family: GH42
PROPERTIES
1.ELECTROPHORETIC PURITY
-Single band on SDS-gel electrophoresis (MW ~77.16 kDa)
Figure 1. Electrophoresis analysis of PpGal42C. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, PpGal42C purified from Ni sepharose fastflow column.
2.SPECIFIC ACTIVITY
1.24 U/mg protein (on pNP-β-gal) at pH 6.0 and 30°C
One Unit of pNP-β-gal activity is defined as the amount of enzyme required to release 1 μmol of p-nitrophenyl per minute from pNP-β-gal (5 mM) inacetate buffer (20 mM) pH 6.0.
3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES
Table 1. Relative activity of PpGal42C on different substratesa.
Substrateb |
Relative activity (%)c |
pNPβGlu |
_ |
pNPβGal |
100.0±0.6 |
pNPβMan |
_ |
pNPβXyl |
_ |
pNPαGlc |
_ |
pNPαGal |
_ |
pNPαMan |
_ |
pNPαAraf |
_ |
pNPαArap |
94.3±0.6 |
aReactions were performed with 5 mM substrate, pH 6.0, at 30°C for 5 min.
bAbsorption caused by released p-nitrophenol was measured at 405 nm. The relative activity on pNPβgal was taken as 100%.
cThe data are reported as means±standard errors from the mean for three independent experiments.
4.PHYSICOCHEMICAL PROPERTIES
pH Optima: 6.0
Temperature Optima: 30°C
5.STORAGE CONDITIONS
The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in acetate buffer (20 mM) pH 6.0. Swirl to mix the enzyme immediately prior to use.
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