BtGal53A（endo-β-1,4-Galactanase）

BtGal53A

Endo-Gal0060

(EC.3.2.1.89)endo-β-1,4-Galactanase

CAZy Family: GH53


PROPERTIES

1.ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~40kDa)

Figure 1. Electrophoresis analysis of BtGal53A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BtGal53A purified from Ni sepharose fastflow column.


2.SPECIFIC ACTIVITY



3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Fig. 2 Depolymerization of pectins at the cell surface of B. thetaiotaomicron cell surface.


4.PHYSICOCHEMICAL PROPERTIES

pH Optima: 7.5

Temperature Optima:37°C



5.STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in sodium

phosphate (20 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.


6. REFERENCES

[1] Luis AS, Briggs J, Zhang X, et al. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219.

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BtGal53A（endo-β-1,4-Galactanase）

BtGal53A

Endo-Gal0060

(EC.3.2.1.89)endo-β-1,4-Galactanase

CAZy Family: GH53


PROPERTIES

1.ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~40kDa)

Figure 1. Electrophoresis analysis of BtGal53A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BtGal53A purified from Ni sepharose fastflow column.


2.SPECIFIC ACTIVITY



3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Fig. 2 Depolymerization of pectins at the cell surface of B. thetaiotaomicron cell surface.


4.PHYSICOCHEMICAL PROPERTIES

pH Optima: 7.5

Temperature Optima:37°C



5.STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in sodium

phosphate (20 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.


6. REFERENCES

[1] Luis AS, Briggs J, Zhang X, et al. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219.