BtuGalA105B (unsaturated galacturonyl hydrolase)
BtuGalA105B
Exo-uGalA0224
(EC.3.2.1.173) unsaturated galacturonyl hydrolase
CAZy Family: GH105
PROPERTIES
1. ELECTROPHORETIC PURITY
-Single band on SDS-gel electrophoresis (MW ~46 kDa)
Figure 1. Electrophoresis analysis of BtuGalA105B. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BtuGalA105B purified from Ni sepharose fastflow column.
2. SPECIFIC ACTIVITY
Displayed activity on uGalAn (n=2 and 3) which was visualized by TLC. A complete loss of the uGalA2 is accompanied by the presence of a new GalA band.
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3. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES
Figure 2. Profiles of oligogalacturonide digestion by GH105 (BtHgl105B).
4. PHYSICOCHEMICAL PROPERTIES
pH Optima: suggested use 4.0
Temperature Optima: suggested use 37°C
5. STORAGE CONDITIONS
The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in 20 mM acetate (pH4.0). Swirl to mix the enzyme immediately prior to use.
6. REFERENCE
[1] Farnell, Benjamin J. Understanding the recognition and utilization of homogalacturonan by Bacteroides thetaiotaomicron[D], 2014
