BtGalA28A

Endo-GalA0223

(EC.3.2.1.82)  exo-galacturonosidase

CAZy Family: GH28

PROPERTIES

1. ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~58 kDa)

Figure 1. Electrophoresis analysis of BtGalA28A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BtGalA28A purified from Ni sepharose fastflow column.

2. SPECIFIC ACTIVITY

 GalA and GalA2 products observed from BT4123 digestion of GalAn

3. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Figure 2. Profiles of oligogalacturonide digestion by BtGalA28A.

4. PHYSICOCHEMICAL PROPERTIES

pH Optima: suggested use 8.0

Temperature Optima: suggested use 37°C

5. STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in 20 mM tris (pH 8.0). Swirl to mix the enzyme immediately prior to use.

6.   REFERENCE

[1] Farnell, Benjamin J. Understanding the recognition and utilization of homogalacturonan by Bacteroides thetaiotaomicron[D], 2014