GeGlu13A

Exo-Glu0002

(EC. 3.2.1.3) exo-α-1,4-Glucosidase

CAZy Family: GH13

PROPERTIES

1. ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~65 kDa)

Figure 1. The purification of recombinant GeGlu13A. Lane M, molecular weight markers; Lane 1, E. coli BL21-pET28a; Lane 2, crude enzyme; Lane 3, heat treatment; Lane 4, purified GeGlu13A.

2. SPECIFIC ACTIVITY

283 U/mg protein (on pNP-α-glu) at pH 7.0 and 65°C.

One Unit of glucosidase activity is defined as the amount of enzyme required to release 1 μmol of pNP per minute from pNP-α-glu in pH 7.0 buffer (20 mM) .

3. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Figure 2. Identification of the hydrolysis products by HPAEC-PAD-200. The hydrolysis products of soluble starch by commercial amylase and recombinant enzyme.

Figure 3. Identification of the transglycosylation products by HPAEC-PAD-200

4. PHYSICOCHEMICAL PROPERTIES

pH Optima: 7.0

pH Stability: 6.0-10.0

Temperature Optima: 65°C

5. STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in acetate buffer (20 mM) pH 4.0. Swirl to mix the enzyme immediately prior to use.

6. REFERENCES

[1] Zhang F, Yuan Y, et al. Heterologous Expression of a Thermostable α-Glucosidase from Geobacillus sp. Strain HTA-462 by Escherichia coli and Its Potential Application for Isomaltose–Oligosaccharide Synthesis. Molecules. 2019, 24, 1413.