PcMan76A

Endo-Man0127

(EC.3.2.1.101) endo-α-1,6-Mannanase

CAZy Family: GH76

PROPERTIES

1.ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~50kDa)

Figure 1. Electrophoresis analysis of PcMan76A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, PcMan76A purified from Ni sepharose fastflow column.

2. PHYSICOCHEMICAL PROPERTIES

pHSuggestion: 7.0

Temperature Suggestion: 37°C

3. STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH7.0. Swirl to mix the enzyme immediately prior to use.