BtAra43B

Ex-Ara0137

(EC.3.2.1.99) endo-α-1,5-Arabinanase

CAZy Family: GH43

PROPERTIES

1.ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~68 kDa)

2.SPECIFIC ACTIVITY

The pNP-α-araf activity was not detected.

3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

^aReactions were performed with 10μg substrate, pH 6.0, at 37°C for 24 h.

^bThe green five pointed star represents arabinose and the orange five pointed star represents xylose.

4.PHYSICOCHEMICAL PROPERTIES

pH Optima: 7.5

Temperature Optima: 37°C

5.STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (50 mM) pH 6.0. Swirl to mix the enzyme immediately prior to use.

6. REFERENCES

[1] Ana S. Luis, Jonathon Briggs, Xiaoyang Zhang, et al. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides Nat Microbiol. 2018,3(2): 210–219.