PcAra43A

Ex-Ara0132

(EC.3.2.1.55) exo-α-1,3-Arabinofuranosidase

CAZy Family: GH43

PROPERTIES

1. ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~50kDa)

Figure 1. Electrophoresis analysis of PcAra43A. lane 1, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 2, PcAra43A purified from Ni sepharose fastflow column.

2. SPECIFIC ACTIVITY

0.42 U/mg protein (on pNP-α-araf) at pH 7.0 and 37°C

One Unit of pNP-α-araf activity is defined as the amount of enzyme required to release 1 μmol of glucose per minute from pNP-α-araf (5 mM) in phosphate buffer(50 mM) pH 7.0

3. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Figure 2. TLC analyses of biotransformation of Rb1, Rb2, Rb3, Rc and Rd by recombinant PcAra43A. 

4. PHYSICOCHEMICAL PROPERTIES

pH Optima: 5.0-6.0

pH Stability: 5.0-9.0

Temperature Optima: 20°C

Temperature Stability: <30°C

5. STORAGE CONDITIONS

The enzyme should be stored at -20℃. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.

6. REFERENCES

[1]Shinozaki, Ayaka, et al. Identification and characterization of three Penicillium chrysogenum α-L-arabinofuranosidases (PcABF43B, PcABF51C, and AFQ1) with different specificities toward arabino-oligosaccharides. Enzyme and microbial technology, 2015, 73, 65-71.