BoAra51B

Ex-Glu0159

(EC.3.2.1.55) exo-α-L-Arabinofuranosidase

CAZy Family: GH51

PROPERTIES

1. ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~78kDa)

Figure 1. Electrophoresis analysis of BoAra51B. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, BoAra51B purified from Ni sepharose fastflow column.

2. SPECIFIC ACTIVITY

0.04 U/mg protein (on pNP-α-araf) at pH 7.0 and 37℃

One Unit of pNP-α-Araf activity is defined as the amount of enzyme required to release 1 μmol of p-nitrophenyl per minute from pNP-α-Araf (5 mM) in phosphate buffer (20 mM) pH 7.0.

3. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Figure 2. TLC analyses of biotransformation of Rb1,Rb2,Rb3,Rc and Rd by recombinant BoAra51B.

4. STORAGE CONDITIONS

The enzyme should be stored at -20℃. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.