PoAra43B

Ex-Ara00344

(EC.3.2.1.99) endo-α-1,5-Arabinanase

CAZy Family: GH43

PROPERTIES

1. ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~36 kDa)

Figure 1. Electrophoresis analysis of PoAra43B. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before Methanol induction; lane 2-4, culture lysat after Methanol induction; lane 5, PoAra43B purified from Ni sepharose fastflow column.

2. SPECIFIC ACTIVITY

0.11 U/mg protein (on Sugar Beet Linear Arabinan) at pH 5.0 and 37°C

One Unit of arabinanase activity is defined as the amount of enzyme required to release 1 μmol of reducing sugar per minute from Sugar Beet Linear Arabinan(2 mg/ml) in Na-Acetate buffer (20 mM) pH 5.0.

3. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Figure 2. Release of reducing sugar from arabinan substrates by PoAra43B.

Figure 3. TLC analyses of the enzymatic hydrolysis products generated from arabino-oligosaccharides (AOS: AA, AAA, AAAA, AA³A, AAA³A, AA²⁺³A), arabinoxylan-oligosaccharides (AXOS: A³X, A²XX, A²⁺³XX, XA²XX, XA³XX) by PoAra43B.

4. STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in acetate buffer (20 mM) pH 4.0. Swirl to mix the enzyme immediately prior to use.