CsXyl43A

Ex-Xyl0167

(EC.3.2.1.37)   exo-β-1,4-xylosidase

CAZy Family: GH43

PROPERTIES

1. ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~56kDa)

Figure 1. Electrophoresis analysis of CsXyl43A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, CsXyl43A purified from Ni sepharose fastflow column.

2. PHYSICOCHEMICAL PROPERTIES

pH Optima: 3.5

pH Stability: 3.0-8.0

Temperature Optima: 80°C

Temperature Stability: 50°-70°C

Figure 2. Effects of pH (A and B) and Temperature(C and D) on Xylosidase Activity and Stability.

3. STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in 20 mM Tris-HCl buffer (pH 8.0). Swirl to mix the enzyme immediately prior to use.

4. REFERENCE

[1] SURYANI, KIMURA T, SAKKA K, et al. Sequencing and expression of the gene encoding the Clostridium stercorarium β-xylosidase Xyl43B in Escherichia coli. Bioscience, biotechnology, and biochemistry, 2004, 68(3): 609-614.