BoXyl43B

Ex-Xyl0168

(EC.3.2.1.37)  exo-β-1,4-xylosidase

CAZy Family: GH43

PROPERTIES

1. ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~37 kDa)

Figure 1. Electrophoresis analysis of BoXyl43B. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BoXyl43B purified from Ni sepharose fastflow column.

2. SPECIFIC ACTIVITY

4.19U/mg protein (on pNP-β-xyl) at pH7.0 and 37°C

One Unit of pNP-β-xyl activity is defined as the amount of enzyme required to release one μmole of xylose per minute from pNP-β-xyl in 50 mM phosphate, pH 7.0.

3. PHYSICOCHEMICAL PROPERTIES

pH Optima: 6.0

Temperature Optima: 25°C

4. STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (50 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.

5. REFERENCE

[1] Jordan D B, Stoller J R, Lee C C, et al. Biochemical characterization of a GH43 β-xylosidase from Bacteroides ovatus. Applied biochemistry and biotechnology, 2017, 182: 250-260.