TbRha78A

Exo-Rha0197

(EC.3.2.1.40) exo-α-1,2- Rhamnosidase

CAZy Family: GH78

PROPERTIES

1. ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~104 kDa)

Figure 1. Electrophoresis analysis of TbRha78A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, TbRha78A purified from Ni sepharose fastflow column.

2. SPECIFIC ACTIVITY

1.52 U/mg protein (on pNP-α-Rha) at pH 7.0 and 37°C.

One Unit of pNP-α- Rha activity is defined as the amount of enzyme required to release 1 μmol of p-nitrophenyl per minute from pNP-α- Rha (5 mM) in phosphate buffer (20 mM) pH 7.0.

3. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Table 1. Relative activity of TbRha78A on different substrate.

4. PHYSICOCHEMICAL PROPERTIES

pH Optima: suggested use 7.0

Temperature Optima: suggested use 37°C

5. STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH 4.0. Swirl to mix the enzyme immediately prior to use.

6. REFERENCES

[1] Hákon B, Gudmundur O, Olafur H. Two new thermostable α-l-rhamnosidases from a novel thermophilic bacterium . Enzyme and Microbial Technology, 2004, 34, 561-571.