BtAra127A(BT0349)

Ex-Ara0140

(EC3.2.1.185)exo-β-Arabinofuranosidase

CAZy Family: GH127

PROPERTIES

1.ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~89 kDa)

Figure 1. Electrophoresis analysis of BtAra127A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BtAra127A purified from Ni sepharose fastflow column.

2.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Table2.Kinetics of BtAra128A

3.PHYSICOCHEMICAL PROPERTIES

pH Optima: 7.5

Temperature Optima: 37°C

4.STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.

5. REFERENCES

[1]Cartmell A, Muñoz-Muñoz J, Briggs J A, et al. A surface endogalactanase in Bacteroides thetaiotaomicron confers keystone status for arabinogalactan degradation. Nature microbiology, 2018, 3(11): 1314-1326.