BtGlu3B

Ex-Glu0207

(EC.3.2.1.21) exo-β-Glucosidase

CAZy Family: GH3

PROPERTIES

1.ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~84 kDa)

Figure 1. Electrophoresis analysis of BtGlu3B. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BtGlu3B purified from Ni sepharose fastflow column.

2.PHYSICOCHEMICAL PROPERTIES

pH suggestion: 7.0

Temperature suggestion: 37°C

3.STORAGE CONDITIONS

The enzyme should be stored at -20 °C. For assay, this enzyme should be diluted in sodiumphosphate (20 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.