BfGlu16A（exo-β-Glucosidase）
BfGlu16A
Ex-Glu0237
(EC.3.2.1.21) exo-β-Glucosidase
CAZy Family: GH16
PROPERTIES
1. ELECTROPHORETIC PURITY
-Single band on SDS-gel electrophoresis (MW ~32kDa)
Figure 1. Electrophoresis analysis of BfGlu16A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, BfGlu16A purified from Ni sepharose fastflow column.
2. SPECIFIC ACTIVITY
0.03 U/mg protein (on pNP-β-glu) at pH 7.0 and 37℃
One Unit of pNP-β-glu activity is defined as the amount of enzyme required to release 1 μmol of p-nitrophenyl per minute from pNP-β-glu (5 mM) in phosphate buffer (20 mM) pH 7.0.
3. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES
Table 1. Relative activity of BfGlu16A on different substrates.
substrates |
linkage |
Activity (μmol/min/mg) |
Relative activity(%) |
Hydroxypropyl cellulose |
β-1,4 |
0.011 |
100.0 |
lichenan |
β-1,3 β-1,4 |
0.005 |
47.5 |
MCC |
β-1,4 |
0.005 |
45.8 |
AHEP-A-6 |
β-1,6 |
0.003 |
23.7 |
Laminaran |
β-1,3 β-1,6 |
0.001 |
5.1 |
β-1,3-Glucan |
β-1,3 |
0.000 |
0.0 |
CMC-Na |
β-1,4 |
0.000 |
0.0 |
Figure 2. TLC analyses of biotransformation of Rb1, Rb2, Rb3, Rc and Rd by recombinant BfGlu16A.
4. STORAGE CONDITIONS
The enzyme should be stored at -20℃. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.
