BoGlu3A

Ex-Glu0291

(EC.3.2.1.21) exo-β-Glucosidase

CAZy Family: GH3

PROPERTIES

1. ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~88kDa)

Figure 1. Electrophoresis analysis of BoGlu3A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, BoGlu3A purified from Ni sepharose fastflow column.

2. SPECIFIC ACTIVITY

25.1 U/mg protein (on pNP-β-glu) at pH 7.0 and 37℃

One Unit of pNP-β-glu activity is defined as the amount of enzyme required to release 1 μmol of p-nitrophenyl per minute from pNP-β-glu (5 mM) in phosphate buffer (20 mM) pH 7.0.

3. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Figure 2. TLC analyses of biotransformation of Rb1,Rb2,Rb3,Rc and Rd by recombinant BoGlu3A.

4. STORAGE CONDITIONS

The enzyme should be stored at -20℃. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.