PpGal42C

Exo-Gal0250

(EC.3.2.1.23)exo-β-Galactosidase

CAZy Family: GH42

PROPERTIES

1.ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~76 kDa)

Figure 1. Electrophoresis analysis of PpGal42C. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, PpGal42C purified from Ni sepharose fastflow column.

2.SPECIFIC ACTIVITY

1.16 U/mg protein (on pNP-β-gal) at pH 7.0 and 37°C.

One Unit of pNP-β-gal activity is defined as the amount of enzyme required to release 1 μmol of p-nitrophenyl per minute from pNP-β-gal(5 mM) in phosphate buffer (20 mM) pH 7.0.

3.RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Table 1. Relative activity of PpGal42C on different substrates.

4.PHYSICOCHEMICAL PROPERTIES

pH Optima: 8.0

Temperature Optima: 40°C

5.STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.