BtGal16A

Endo-Gal0078

(EC.3.2.1)endo-β-Galactanase

CAZy Family: GH16

PROPERTIES

1.ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~29 kDa)

Figure 1. Electrophoresis analysis of BtGal16A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BtGal16A purified from Ni sepharose fastflow column.

2.  RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Figure 2 TLC of BtGal16A hydrolyzed lactose. The concentration of lactose was 2 mg/mL in all reactions. The reactions were incubated in 20 mM sodium phosphate buffer (pH 7.0) for 12 hours.

3.PHYSICOCHEMICAL PROPERTIES

pH Optima: suggested use 7.0

Temperature Optima: suggested use 37°C

4.STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.