BvMan2A

Ex-Man0111

(EC.3.2.1.25)  exo-β-Mannosidase

CAZy Family：GH2

PROPERTIES

1. ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~99 kDa)

Figure 1. Electrophoresis analysis of BvMan2A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate before IPTG induction; lane 2, culture lysate after IPTG induction; lane 3, BvMan2A purified from Ni sepharose fastflow column.

2. SPECIFIC ACTIVITY

0.47 U/mg protein (on pNP-β-Man) at pH 7.0 and 37°C.

One Unit of pNP-β-Man activity is defined as the amount of enzyme required to release 1 μmole of p-nitrophenyl per minute from pNP-β-Man (5 mM) in phosphate buffer (20 mM) pH 7.0.

3. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

Table 1. Relative activity of BvMan2A on different substrates.

4. PHYSICOCHEMICAL PROPERTIES

pH Optima: Suggested use 7.0

Temperature Optima: Suggested use 37°C

5. STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (20 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.